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Related Experiment Video

Updated: Mar 7, 2026

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

Technological advances for interrogating the human kinome.

Akanksha Baharani^1,2, Brett Trost³, Anthony Kusalik⁴

¹Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada.

Biochemical Society Transactions

|February 17, 2017

Summary

This summary is machine-generated.

Kinome analysis offers insights into cell biology and disease by examining cellular kinase activity. This review covers advancements in kinome profiling technologies and remaining challenges for high-throughput analysis.

Keywords:

DAPPLE PIIKA PamChip kinase kinome peptide arrays

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Area of Science:

Biochemistry
Molecular Biology
Cellular Signaling

Background:

Increasing recognition of cellular kinase (kinome) activity's importance in understanding biology and disease.
Kinome analysis aids in understanding complex biological processes, including disease pathology.
Kinome profiling is crucial for identifying biomarkers and therapeutic targets.

Purpose of the Study:

To review recent technological advancements in kinome profiling.
To provide perspectives on the challenges and future directions in high-throughput kinome analysis.
To highlight the significance of phosphorylation-mediated signaling events.

Main Methods:

Review of current technologies for kinome profiling.
Analysis of methods for characterizing phosphorylation-mediated signaling.
Discussion of cost-effective, high-throughput approaches.

Main Results:

Recent advances in technologies enable detailed kinome profiling.
High-throughput methods are being optimized for cost-effectiveness.
Key challenges in kinome analysis are identified.

Conclusions:

Technological progress is enhancing kinome profiling capabilities.
Further development is needed to address remaining challenges in high-throughput analysis.
Kinome profiling remains a vital tool for biological and clinical research.