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Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice
Maaike Kockx1, Wendy Jessup2, Leonard Kritharides2
1ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, 2139, Australia. maaike.kockx@sydney.edu.au.
Methods in Molecular Biology (Clifton, N.J.)
|February 17, 2017
Abstract:
Reverse cholesterol transport (RCT) is one of the main processes that is thought to protect against cardiovascular disease. RCT constitutes the removal of cholesterol from peripheral sites, its transport through the plasma compartment for delivery to the liver for excretion. Here, we describe an in vivo RCT method that incorporates these steps, measuring movement of cholesterol from macrophages to the plasma, the liver, and finally to the feces in mice.
