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Related Experiment Video

Updated: Mar 7, 2026

Author Spotlight: Isolating Biomolecules from Mouse Tears — A Methodology for Molecular Analysis and Biomarker Research

Author Spotlight: Isolating Biomolecules from Mouse Tears — A Methodology for Molecular Analysis and Biomarker Research

Published on: July 19, 2024

Paper-based microfluidic system for tear electrolyte analysis.

Ali K Yetisen¹, Nan Jiang², Ali Tamayol¹

¹Biomaterials Innovation Research Center, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA. akyetisen@gmail.com and Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. syun@mgh.harvard.edu.

|February 17, 2017

Summary

This summary is machine-generated.

A new portable microfluidic system enables quantitative electrolyte analysis in tears at the point-of-care. This technology aids in early dry eye disease diagnosis and subtyping, improving ocular disorder management.

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Published on: July 19, 2024

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

Biomedical Engineering
Ophthalmology
Analytical Chemistry

Background:

Point-of-care tear analysis offers potential for early ocular disorder diagnosis.
Current methods are limited to osmolarity/inflammatory markers and cannot differentiate dry eye subtypes.

Purpose of the Study:

To develop a portable microfluidic system for quantitative electrolyte analysis in tear fluid.
To enable point-of-care differentiation and staging of dry eye disease subtypes.

Main Methods:

A microfluidic system incorporating a capillary tube, dilution reservoir, and paper-based device.
Fluorescent crown ethers, o-acetanisidide, and seminaphtorhodafluor for electrolyte sensing.
Smartphone readout for fluorescence measurement of Na+, K+, Ca2+ ions and pH.

Main Results:

Accurate measurement of Na+, K+, Ca2+ ions and pH in artificial tears within physiological ranges.
Demonstrated feasibility for detecting early-stage dry eye disease.
Showcased potential for differential diagnosis and severity staging of dry eye subtypes.

Conclusions:

The developed portable microfluidic system enables quantitative tear electrolyte analysis.
This technology supports point-of-care diagnosis, subtyping, and staging of dry eye disease.
Offers a low-cost, rapid screening tool for ocular disorders.