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Updated: Aug 10, 2026
Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
Published on: March 5, 2013
SGHTL-34, a thyrotrophin-responsive immortalised human thyroid cell line generated by transfection
G S Whitley1, S S Nussey, A P Johnstone
1Department of Immunology, St. George's Hospital Medical School, London, U.K.
Abstract:
Normal human thyrocytes were immortalised by electroporation-mediated transfection with the plasmid pSV3neo. One resultant cell line (SGHTL-34) contains approximately 10 copies of simian virus 40 (SV40) early region incorporated into the genome and has been in continuous monolayer culture for 9 months. SGHTL-34 cells grow rapidly (doubling time 30 h) and contain cytokeratin filaments. They demonstrate a morphological change in response to thyrotrophin (TSH) and possess a TSH- and forskolin-sensitive adenylate cyclase, the thresholds for stimulation being 10 microU/ml bovine TSH- and forskolin-sensitive development of this novel human thyrocyte cell line may allow further study of the regulation of human thyroid growth and differentiation.
