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Effect of vitamin E on FMLP-induced activation of rabbit polymorphonuclear leukocytes
S J Weisman1, J E Lafuze, R A Haak
1Department of Pediatrics, University of Connecticut Health Center, Farmington 06032.
Abstract:
Granulocytes of vitamin E-treated rabbits were compared to granulocytes from placebo-treated rabbits. Granulocytes were isolated from rabbit peripheral blood by a new method employing Percoll and gelatin sedimentation. Vitamin E-treated cells showed less adherence to rabbit aortic endothelium when stimulated with FMLP. FMLP receptor numbers and affinity were not significantly different. Resting cell surface and baseline transmembrane potential were similar in both cell types. Decrease in cell surface potential with FMLP was comparable in vitamin E- and placebo-treated cells. Vitamin E-treated PMN depolarized more and hyperpolarized more rapidly than placebo cells. Thus vitamin E-treated PMNs show differences in the early events of PMN activation. These may contribute to the lower stimulated adherence observed with vitamin E-treated cells.
Insights
Vitamin E treatment reduced granulocyte adherence to endothelium in rabbits. These vitamin E-treated neutrophils (PMNs) showed altered early activation events, potentially explaining reduced adherence.
Area of Science:
- Immunology
- Cell Biology
- Nutritional Science
Background:
- Granulocyte adherence to endothelium is a key inflammatory process.
- Vitamin E is an antioxidant with potential immunomodulatory effects.
Purpose of the Study:
- To investigate the effect of vitamin E on rabbit granulocyte function.
- To compare vitamin E-treated granulocytes with placebo-treated controls.
Main Methods:
- Isolation of granulocytes from rabbit peripheral blood using Percoll and gelatin sedimentation.
- Assessment of granulocyte adherence to rabbit aortic endothelium upon stimulation with FMLP.
- Measurement of FMLP receptor characteristics and cell membrane potential changes.
Main Results:
- Vitamin E-treated granulocytes exhibited significantly less adherence to endothelium when stimulated.
- No significant differences were found in FMLP receptor number or affinity.
- Vitamin E-treated PMNs showed altered depolarization and hyperpolarization dynamics during activation.
Conclusions:
- Vitamin E influences early activation events in rabbit granulocytes (PMNs).
- These alterations in cellular response may underlie the observed reduction in stimulated adherence.
- Vitamin E may play a role in modulating inflammatory cell behavior.
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