Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

27.9K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.9K
CRISPR01:59

CRISPR

58.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.5K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.9K
Overview
80.9K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

1.3K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.3K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.3K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.3K
RNA Editing02:23

RNA Editing

10.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic risk prediction of type 2 diabetes in people living with and without HIV.

Scientific reports·2026
Same author

Interleukin-2 is a potent latency reversal agent in people with treated HIV-1.

Science advances·2025
Same author

Age modifies the association between sex and the plasma inflammatory proteome in treated HIV.

The Journal of clinical investigation·2025
Same author

Sex differences in HIV-1 reservoir cell selection are linked to altered innate immune profiles.

Science translational medicine·2025
Same author

CRISPR antiviral inhibits neurotrophic JC polyomavirus in 2D and 3D culture models through dual-gRNA excision by SaCas9.

Molecular therapy. Nucleic acids·2025
Same author

Burden of Chronic Health Conditions Among People With HIV and Common Non-AIDS-Defining Cancers.

Journal of the National Comprehensive Cancer Network : JNCCN·2025

Related Experiment Video

Updated: Mar 7, 2026

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
12:03

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

8.3K

Novel AIDS therapies based on gene editing.

Kamel Khalili1,2,3, Martyn K White4,5, Jeffrey M Jacobson4,5,6,7

  • 1Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N. Broad Street, 7th Floor, Philadelphia, PA, 19140, USA. kamel.khalili@temple.edu.

Cellular and Molecular Life Sciences : CMLS
|February 18, 2017
PubMed
Summary

Gene editing offers a promising strategy to eliminate latent HIV by targeting infected cells and receptors, potentially leading to a cure for AIDS. This approach aims to overcome limitations of current "shock and kill" methods.

Keywords:
AIDSCRISPR/Cas9Gene editingHIV-1

More Related Videos

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

5.4K
Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices
07:43

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

Published on: July 14, 2023

2.9K

Related Experiment Videos

Last Updated: Mar 7, 2026

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
12:03

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

8.3K
Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

5.4K
Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices
07:43

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

Published on: July 14, 2023

2.9K

Area of Science:

  • Virology
  • Immunology
  • Gene Therapy

Background:

  • HIV/AIDS remains a significant global health challenge, affecting millions worldwide.
  • Combination antiretroviral therapy (cART) has transformed HIV into a manageable chronic condition, but lifelong treatment is necessary and does not eliminate the virus.
  • Latent HIV reservoirs in cells like CD4+ T cells pose a barrier to a cure, allowing for viral persistence and potential resurgence.

Purpose of the Study:

  • To review gene-editing strategies for targeting and eliminating latent HIV reservoirs.
  • To explore the potential of gene editing in achieving a functional or sterile cure for AIDS.

Main Methods:

  • Inactivation of integrated proviral DNA within the host cell genome using gene-editing tools.
  • Disruption of HIV receptors on host cells to prevent viral entry and replication.
  • Review of recent advances in gene-editing technologies applicable to HIV cure strategies.

Main Results:

  • Gene editing shows potential for precise targeting of latent HIV provirus.
  • Knocking out HIV receptors can prevent viral spread and reactivation.
  • These approaches offer an alternative to traditional "shock and kill" methods with potential for greater efficacy.

Conclusions:

  • Gene editing represents a novel and promising therapeutic avenue for eradicating latent HIV reservoirs.
  • This technology holds the potential to achieve a sterile cure for AIDS, moving beyond viral suppression.
  • Further research and clinical assessment are needed to fully realize the therapeutic potential of gene editing in HIV treatment.