Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>Sleeping Beauty</i> mutagenesis identifies <i>BACH2</i> and other regulators of CD8<sup>+</sup> T-cell exhaustion, persistence in vivo, and CAR-T cell function under tumor-associated chronic antigen stimulation.

Journal for immunotherapy of cancer·2026

Same author

Uncovering Sex Differences in the Drosophila Ventral Nerve Cord Through Connectome Alignment.

bioRxiv : the preprint server for biology·2026

Same author

Cryopreservation and other preservation approaches for animal models workshop session I: Invertebrate models in biomedical research.

Cryobiology·2026

Same author

Inhibitory columnar feedback neurons are involved in motion processing in <i>Drosophila</i>.

eLife·2026

Same author

Navigation circuits: Calcium spikes know which way the wind blows.

Current biology : CB·2026

Same author

Octopamine instructs head direction plasticity.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: Mar 7, 2026

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Published on: June 3, 2011

FlpStop, a tool for conditional gene control in Drosophila.

Yvette E Fisher¹, Helen H Yang¹, Jesse Isaacman-Beck¹

¹Department of Neurobiology, Stanford University, Stanford, United States.

|February 18, 2017

Summary

FlpStop enables precise gene disruption in non-dividing cells, advancing studies of gene function in Drosophila. This tool facilitates research into neural circuits and behavior by enabling conditional gene manipulation.

Keywords:

D. melanogaster apterous chromosomes conditional gene disruption conditional gene rescue genes neuroscience voltage-gated calcium channels

More Related Videos

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Published on: October 6, 2016

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

Related Experiment Videos

Last Updated: Mar 7, 2026

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Published on: June 3, 2011

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Published on: October 6, 2016

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

Area of Science:

Developmental Biology
Neuroscience
Genetics

Background:

Cell type-specific gene manipulation is crucial for understanding gene function in Drosophila research, particularly in neural development, circuit computation, and behavior.
Existing techniques for cell type-specific gene disruption in flies often result in incomplete gene activity reduction or depend on cell division, limiting their application in post-mitotic cells.

Purpose of the Study:

To introduce FlpStop, a versatile tool for conditional gene disruption and rescue in post-mitotic cells.
To demonstrate the efficacy of FlpStop through manipulation of key genes involved in development and neuronal function.

Main Methods:

Development of FlpStop, a generalizable tool for conditional gene disruption and rescue in post-mitotic cells.
Proof-of-principle experiments involving the manipulation of apterous, a wing development regulator.
Creation of conditional null alleles for Glutamic acid decarboxylase 1 (Gad1), Resistant to dieldrin (Rdl), cacophony (cac), and paralytic (para) genes.
Application of FlpStop to manipulate cac in a specific visual interneuron type.

Main Results:

Successful demonstration of FlpStop's capability for conditional gene disruption and rescue in post-mitotic cells.
Validation of FlpStop through manipulation of developmental and neuronal genes, including those essential for GABAergic neurotransmission and neuronal excitability.
Discovery of differential regulation of calcium signals across subcellular compartments in a specific visual interneuron type upon cac manipulation.

Conclusions:

FlpStop provides a powerful and generalizable method for conditional gene manipulation in post-mitotic cells.
This tool will significantly facilitate investigations into the complex interactions between genes, neural circuits, and computation.
FlpStop opens new avenues for studying gene function in the nervous system and other post-mitotic tissues in Drosophila.