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Quantitative immunocytochemical assay for infectious avian retroviruses
1Division of Biology and Medicine, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
The Journal of General Virology
|September 1, 1987
Abstract:
A simple and accurate immunocytochemical focus assay is described, whereby both transforming and non-transforming avian retroviruses can be enumerated. After virus infection of chick embryo fibroblasts, an agar overlay is applied; foci of infected cells (expression foci) are detected immunocytochemically after 5 to 7 days. The primary antibodies are monoclonal sera directed against either viral p19gag or pp60v-src. Detection of expression foci after transfection of cells with cloned viral DNA is also demonstrated.