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Updated: Mar 7, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Structural analysis of a homogeneous polysaccharide from Achatina fulica
Jie Liu1, Feineng Shang2, Zengming Yang3
1State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Pharmaceutical Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; School of Pharmacy, Yunnan University of TCM, Kunming 650500, China.
Abstract:
Edible snails have been widely used as a health food and medicine in many countries. In our study, a water-soluble polysaccharide (AF-1) was isolated and purified from Achatina fulica by papain enzymolysis, alcohol precipitation and strong anion exchange chromatography. Structureof the polysaccharide was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, high performance liquid chromatography, analysis of monosaccharide composition, methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy (1H, 13C, COSY, TOCSY, NOESY, HSQC and HMBC). Chemical composition analysis indicated that AF-1 is composed of glucose (Glc) and its average molecular weight is 1710kDa. Structural analysis suggested that AF-1 is mainly consisted of a linear repeating backbone of (1→4) linked α-d-Glc p residues with one branch, α-d-Glc p, attached to the main chain by (1→6) glycosidic bonds at every five main-chain units. Further studies on biological activities of the polysaccharide are currently in progress.
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