Related Experiment Video
Updated: Mar 7, 2026
10:30
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
8.3K
Dynamic flow-through approach to evaluate readily bioaccessible antioxidants in solid food samples.
Miguel A Maia1, Tânia R P Soares1, Ana I P Mota1
1UCIBIO, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Talanta
|February 19, 2017
Summary
This study developed an affordable flow-through device for dynamic leaching experiments to assess readily bioaccessible antioxidant compounds in foods. The method accurately estimates antioxidant bioavailability, correlating well with traditional batch extraction techniques.
Area of Science:
- Food Science
- Analytical Chemistry
- Bioavailability Studies
Background:
- Bioactive compound release from food is crucial for human bioavailability.
- Assessing readily bioaccessible antioxidants in solid foods requires efficient methods.
- Existing methods for antioxidant bioavailability assessment can be time-consuming.
Purpose of the Study:
- To implement an affordable and robust flow-through device for dynamic leaching experiments.
- To assess readily bioaccessible antioxidant compounds in solid food commodities.
- To evaluate the kinetic extraction profile and compare it with conventional methods.
Main Methods:
- Utilized commercially available devices with regenerated cellulose filters for a disposable extraction chamber.
- Employed the ABTS (2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)) assay to evaluate kinetic extraction profiles.
- Fitted a first-order reaction model to determine leaching rate constants and compared results with batch-wise extraction.
Main Results:
- Developed a flow-through device for expedient dynamic leaching experiments.
- Achieved good correlation (R>0.9) with a first-order reaction model for readily bioaccessible compounds.
- Demonstrated no statistically significant differences between kinetic model estimates and conventional batch-wise extraction values for antioxidant content (3.37–60.3 µmol Trolox/g).
- Showcased gastrointestinal compartment-dependent kinetic leaching rates (0.120 and 0.198 min⁻¹) and antioxidant content (45.5 and 52.5 µmol Trolox/g) in spinach using surrogate biological media.
Conclusions:
- The developed flow-through device offers an affordable and robust method for assessing readily bioaccessible antioxidants in solid foods.
- The kinetic model accurately predicts antioxidant bioavailability, aligning with traditional methods.
- The method is adaptable for simulating gastrointestinal conditions to determine antioxidant leaching rates and content.
More Related Videos
Related Concept Videos
01:22Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems
306
Bioavailability is a critical pharmacological concept that measures the extent and rate at which an active drug ingredient or therapeutic moiety enters the systemic circulation, remaining unchanged. It's a pivotal factor in determining a drug's efficacy and safety.The Biopharmaceutics Classification System (BCS) plays an essential role in drug development by categorizing drugs into four classes based on their solubility and permeability. This classification aids in understanding drug absorption...
306
01:20Measurement of Bioavailability: Pharmacodynamic Methods
936
Pharmacodynamic methods provide insights into a drug's effects on physiological processes over time and play a crucial role in understanding bioavailability and therapeutic efficacy. These methods can be broadly classified into acute pharmacological and therapeutic response approaches, each with distinct mechanisms and applications.The acute pharmacological response method directly correlates a drug's physiological effects, such as ECG or pupil diameter changes, to its time course in the body.
936
01:16Methods for Studying Drug Absorption: In vitro
682
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
682
01:30Measurement of Bioavailability: Pharmacokinetic Methods
370
Pharmacokinetics is a vital branch of pharmacology that examines how drugs are absorbed, distributed, metabolized, and excreted by the body. Two key methodologies in pharmacokinetics are plasma drug concentration studies and urinary drug excretion analyses, both of which provide critical insights into a drug's therapeutic efficacy and bioavailability.Plasma Drug Concentration-Time StudiesPlasma drug concentration-time studies involve analyzing blood samples at specific intervals to quantify...
370
01:23Factors Influencing Bioavailability: First-Pass Elimination
8.4K
When a drug is taken orally, it undergoes a journey starting from the gastrointestinal (GI) tract, passing through the portal vein, reaching the liver, and finally entering the systemic circulation. This process involves the absorption of the drug across the GI tract. The liver is the primary site for metabolizing the drug, with some metabolism also occurring in the gut wall. This journey significantly reduces the quantity of the drug that reaches the systemic circulation, a phenomenon known as...
8.4K
01:17In Vitro Drug Dissolution: Alternative Methods
312
Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
312