Related Experiment Video
Updated: Mar 7, 2026

Identification of Plasmodesmal Localization Sequences in Proteins In Planta
Published on: August 15, 2017
Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting
Yufeng Fang1,2, Brett M Tyler1,2
1Interdisciplinary Ph.D. Program in Genetics, Bioinformatics & Computational Biology, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA, 24061, USA.
Abstract:
Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that the oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into the nucleus that differ from conventional well-characterized NLSs from mammals and yeast. Here, we have characterized in depth the NLSs of a P. sojae basic leucine zipper transcription factor, PsbZIP1. Nuclear localization of PsbZIP1 was determined by a central conserved region overlapping the DNA binding domain. Mutational analysis of this region identified four distinct elements that contributed multiplicatively to nuclear localization, but the conserved DNA binding residues were not required. Three of the elements showed autonomous NLS activity and the fourth served as a nuclear localization enhancer. Sequences within two of the nuclear localization elements defined a new form of bipartite NLS consisting of a triplet of basic residues followed by a tail of scattered basic amino acids.
Related Concept Videos
Regulation of Nuclear Protein Sorting
Nuclear Localization Signals and Import
Regulated mRNA Transport
Nuclear Protein Sorting
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Leaky Scanning

