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A multifunctional alanine-rich anti-inflammatory peptide BCP61 showed potent inhibitory effects by inhibiting both

Yun Hee Choi1, Yoon Seok Choi1, Young Kyun Kim1

  • 1Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759, Korea.

Inflammation
|February 20, 2017
PubMed

Insights

BCP61, an antimicrobial peptide, demonstrates significant anti-inflammatory effects by inhibiting key inflammatory pathways and mediators. This suggests BCP61

Area of Science:

  • Biochemistry
  • Immunology
  • Pharmacology

Background:

  • BCP61 is a unique low-molecular-weight antimicrobial peptide.
  • Its N-terminal sequence is rich in alanine and non-identical.

Purpose of the Study:

  • To investigate the anti-inflammatory effects of BCP61.
  • To examine BCP61's impact on inflammatory mediators and signaling pathways in LPS-stimulated Raw 264.7 cells.

Main Methods:

  • Treatment of Raw 264.7 cells with varying concentrations of BCP61 (10, 50, 100 μg/mL).
  • Assessment of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), pro-inflammatory cytokines (TNF-α, IL-6), NF-κB, and MAPKs (ERK, JNK, p38) expression and production.
  • Evaluation of IκBα phosphorylation and degradation, and p65 nuclear translocation.

Main Results:

  • BCP61 inhibited LPS-induced NF-κB and MAPK signaling pathways.
  • BCP61 reduced the production of pro-inflammatory mediators including nitric oxide (NO) and prostaglandin E2 (PGE2).
  • BCP61 prevented IκBα phosphorylation and degradation, inhibiting p65 nuclear translocation.

Conclusions:

  • BCP61 exhibits potent anti-inflammatory properties.
  • BCP61 may serve as a therapeutic candidate for treating inflammation and microbial infections.
Keywords:
MAPKsNF-κBRAW 264.7anti-inflammatory peptide bcp61

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