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Fluorescence eXclusion Measurement of volume in live cells
C Cadart1, E Zlotek-Zlotkiewicz1, L Venkova1
1Institut Curie/Institut Pierre-Gilles de Gennes, Paris, France.
Methods in Cell Biology
|February 21, 2017
Summary
Accurately measuring single living cell volume is now possible with a novel fluorescent dye exclusion method. This versatile technique is compatible with various cell types and standard laboratory tools, advancing cell biology research.
Area of Science:
- Cell biology
- Biophysics
Background:
- Cell volume is a fundamental property, yet precise measurement remains challenging in cell biology.
- Previous studies often relied on indirect proxies like mass or density for cell size estimation.
Purpose of the Study:
- To introduce a reliable method for measuring the volume of single living cells.
- To provide a versatile tool applicable to diverse cell types and experimental conditions.
Main Methods:
- Developed a method based on the principle of fluorescent dye exclusion for cell volume determination.
- Ensured compatibility with standard cell biology laboratory equipment and techniques.
Main Results:
- The method enables precise volume measurements for individual living cells.
- Demonstrated versatility across different cell sizes, states (adherent/suspension), and over multiple cell cycles.
- Confirmed independence from cell shape variations and compatibility with epifluorescence imaging and drug treatments.
Conclusions:
- The fluorescent dye exclusion method offers an accessible and versatile solution for single living cell volume measurement.
- This technique facilitates more accurate investigations into cell volume dynamics and its role in cellular processes.
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