Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Intelligence level and structure in school age children with fetal growth restriction].

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics·2009
Same author

Uncoupling the D1-N-methyl-D-aspartate (NMDA) receptor complex promotes NMDA-dependent long-term potentiation and working memory.

Biological psychiatry·2009
Same author

A phospho-directed macroporous alumina-silica nanoreactor with multi-functions.

ACS nano·2009
Same author

Subintimal angioplasty for below-the-ankle arterial occlusions in diabetic patients with chronic critical limb ischemia.

Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists·2009
Same author

Natural killer/T cell lymphoma initiating with pleural effusion: the significance of MICM combined techniques for the diagnosis.

Zhongguo shi yan xue ye xue za zhi·2009
Same author

[Advance of study on animal models of lymphoma].

Zhongguo shi yan xue ye xue za zhi·2009
Same journal

Dynamic coordination and segregation mechanisms in higher cortex for parallel task processing.

Neuron·2026
Same journal

Higher-order thalamic bursts are drivers of attention control.

Neuron·2026
Same journal

Composing trajectories for rapid inference of navigational goals.

Neuron·2026
Same journal

Peri-head distance coding in the mouse brainstem.

Neuron·2026
Same journal

A two-timepoint framework for sensitive and specific single-cell activity screening.

Neuron·2026
Same journal

From first impressions to bonds: The neural dynamics of social relationships.

Neuron·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States
06:25

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Published on: January 19, 2024

1.7K

Long-Term Two-Photon Imaging in Awake Macaque Monkey.

Ming Li1, Fang Liu1, Hongfei Jiang1

  • 1Peking University School of Life Sciences and Peking-Tsinghua Center for Life Sciences, Beijing 100871, China; IDG/McGovern Institute for Brain Research at Peking University, Beijing 100871, China.

Neuron
|February 21, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed long-term two-photon imaging techniques for awake macaque monkeys. This breakthrough allows detailed study of neuronal circuits and higher cognitive functions using genetic tools.

More Related Videos

Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice
09:34

Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice

Published on: June 19, 2019

17.1K
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
16:45

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Published on: March 13, 2016

12.1K

Related Experiment Videos

Last Updated: Mar 7, 2026

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States
06:25

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Published on: January 19, 2024

1.7K
Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice
09:34

Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice

Published on: June 19, 2019

17.1K
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
16:45

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Published on: March 13, 2016

12.1K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Primate Research

Background:

  • Understanding higher cognitive functions requires studying molecular and neuronal circuits in vivo.
  • Existing imaging techniques in awake primates are limited for long-term, high-resolution studies.

Purpose of the Study:

  • To establish reliable techniques for long-term two-photon imaging in awake macaque monkeys.
  • To enable the use of genetic tools for studying neuronal populations in non-human primates.

Main Methods:

  • Utilized genetically encoded calcium indicators (GCaMP5, GCaMP6s) delivered via Adeno-Associated Virus serotype 2/1 (AAV2/1).
  • Performed long-term two-photon imaging in the visual cortex of awake macaque monkeys.
  • Conducted simultaneous intracellular recording and two-photon calcium imaging.

Main Results:

  • Achieved and maintained high-quality two-photon imaging of large neuronal populations for months.
  • Demonstrated linear proportionality between fluorescence activity and neuronal spiking rates (10-150 Hz).
  • Enabled single-cell and single dendritic spine resolution imaging in awake monkeys.

Conclusions:

  • The developed techniques facilitate fundamental advances in understanding higher cognitive function.
  • This method bridges the gap between genetic tools and the study of complex brain functions in primates.
  • Provides unprecedented access to neuronal circuit dynamics in awake, behaving macaques.