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Free radicals trapped in methyl alpha-D-mannopyranoside X-irradiated at 77 K: an ESR/ENDOR study
W A Bernhard1, J Huttermann, D M Close
1Department of Biophysics, University of Rochester, New York 14642.
Abstract:
Four free radicals are trapped in methyl alpha-D-mannopyranoside X-irradiated and maintained at 77 K. All four have been identified, with high confidence levels, using ESR and ENDOR spectroscopy. One, an alkoxy radical located at O4, is characterized by a gmax of 2.059, an isotropic beta hydrogen hyperfine coupling (hfc) of 98 MHz, and small interactions due to gamma or delta hydrogens. The second, a secondary dioxyalkyl radical due to loss of hydrogen from C1 is characterized by one beta hfc with an isotropic component of 19.03 MHz. The third, a secondary hydroxyalkyl radical due to loss of hydrogen from C2 is characterized by two nonexchangeable hydrogens with isotropic beta interactions of 22.45 and 6.44 MHz and one exchangeable hydrogen with an isotropic beta interaction of 9.88 MHz. The fourth is a .CH2OR radical that is formed by the net loss of hydrogen from the methyl group.