Related Experiment Videos

Structural proteins of bovine parainfluenza-3 virus

P Panigrahi1, S B Mohanty, R K Maheshwari

  • 1Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland Campus, College Park 20742.

Veterinary Microbiology
|March 1, 1987
PubMed

Insights

Researchers identified six structural proteins in bovine parainfluenza-3 virus (PI-3V) using [35S]-methionine labeling and PAGE. A seventh protein, cellular actin, was also detected in this bovine PI-3V study.

Area of Science:

  • Virology
  • Molecular Biology
  • Protein Chemistry

Background:

  • Bovine parainfluenza-3 virus (PI-3V) is a significant pathogen in cattle.
  • Previous studies reported five structural proteins of PI-3V.
  • Understanding viral protein composition is crucial for developing control strategies.

Purpose of the Study:

  • To identify and characterize the structural proteins of bovine parainfluenza-3 virus (PI-3V).
  • To compare the protein profile with previously reported findings.
  • To investigate the presence of specific glycoproteins and associated cellular proteins.

Main Methods:

  • Bovine PI-3V was labeled with [35S]-methionine.
  • Proteins were separated and visualized using polyacrylamide gel electrophoresis (PAGE).
  • Selective labeling with [2-3H]-mannose was employed to identify glycoproteins.

Main Results:

  • Six structural proteins of PI-3V were resolved: L (180 kD), P (83 kD), HN (69 kD), NP (66 kD), F (55 kD), and M (38 kD).
  • Selective mannose labeling confirmed HN and F as glycoproteins.
  • A seventh protein, identified as cellular actin (43 kD), was also found associated with the virus.

Conclusions:

  • This study expands the known structural protein profile of bovine PI-3V.
  • The identification of cellular actin suggests potential host-pathogen interactions.
  • Further research into the function of these proteins can aid in PI-3V vaccine and therapeutic development.

Related Concept Videos