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Related Experiment Videos

A highly sensitive bioassay for PTH using ROS 17/2.8 subclonal cells.

K Sato1, D C Han, M Ozawa

  • 1Institute of Clinical Endocrinology, Tokyo Women's Medical College, Japan.

Acta Endocrinologica
|September 1, 1987
PubMed
Summary

Researchers developed a sensitive bioassay for parathyroid hormone (PTH) using specialized rat osteosarcoma cells. This new method significantly enhances the detection of PTH and related factors, aiding in cancer research.

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Area of Science:

  • Endocrinology
  • Cell Biology
  • Cancer Research

Background:

  • Parathyroid hormone (PTH) plays a crucial role in calcium homeostasis.
  • Malignant tumors can produce PTH-like factors, leading to humoral hypercalcemia.
  • Existing bioassays for PTH may lack the sensitivity to detect tumor-derived factors.

Purpose of the Study:

  • To develop a highly sensitive bioassay for parathyroid hormone (PTH).
  • To improve the detection of PTH-like factors produced by tumors.
  • To aid in the characterization and purification of tumor-derived hypercalcemic factors.

Main Methods:

  • Development of a bioassay using rat osteosarcoma cells (ROS 17/2.8).
  • Subcloning of ROS cells to identify the most PTH-responsive cell line (ROS 17/2.8-5).

Related Experiment Videos

  • Treatment of ROS 17/2.8-5 cells with hydrocortisone to enhance sensitivity.

    • Main Results:

    • Selected subclonal cell line (ROS 17/2.8-5) showed significant cAMP response to PTH.
    • Sensitivity for PTH detection was improved approximately 15-fold with hydrocortisone treatment.
    • The bioassay did not respond to osteoclast activating factors but did respond to a tumor-conditioned medium.

    Conclusions:

    • A highly sensitive bioassay for PTH was successfully developed using hydrocortisone-treated ROS 17/2.8-5 cells.
    • This enhanced bioassay can detect PTH and potentially PTH-like factors from tumors.
    • The method is valuable for characterizing and purifying tumor-derived factors contributing to hypercalcemia.