Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell...

Area of Science:

• Biotechnology
• Cell Biology
• Microfluidics

Background:

• Single cell analysis is increasingly important in academia and clinics.
• Effective upstream cell sample preparation is crucial for single cell analysis.
• High-efficiency cell enrichment and precise single cell capture are key challenges.

Purpose of the Study:

• To review microfluidic technologies for cell enrichment and single cell capture.
• To highlight recent advancements (last five years) in cell manipulation technologies.
• To outline frameworks and showcase applications of these technologies.

Main Methods:

• Review of microfluidic technologies for cell enrichment and capture.
• Focus on recent developments and representative works.
• Categorization of technologies for cell manipulation.

Main Results:

• Microfluidics enables significant advances in cell enrichment and single cell capture.
• Technologies reviewed show promise for both circulating tumor cell (CTC) enrichment and general single cell capture.
• Most technologies are applicable to both cell enrichment and precise single cell capture.

Conclusions:

• Microfluidic technologies offer innovative solutions for cell sample preparation.
• These advancements are transitioning into real clinical applications.
• Ongoing research and development present opportunities for further innovation.