Related Experiment Video
Updated: Mar 7, 2026

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
Published on: May 15, 2021
Captopril apparently increase and cisplatin apparently decrease human albumin concentration in artificial urinary
Rafał Kuzioła1, Barbara Marczewska1, Krzysztof Marczewski2
1Institute of Environmental Engineering, The John Paul II Catholic University of Lublin, Lublin, Poland.
Background:
Measurement of urinary albumin (HSA) is very important in diagnostic of kidney diseases. Much less is known about the possible impact of substances present in urine together with albumin on the results of measurements.
Methods:
We investigated the effect of the presence of captopril and cisplatin in the solution on the result of the determination of HSA by native polyacrylamide gel electrophoresis. Protein conformation in the absence and presence of the drugs was examined using Fourier Transform Infrared Spectroscopy (FTIR).
Results:
The presence of captopril apparently increases HSA concentration while cisplatin causes an apparent decrease in the HSA concentration. The presence of both drugs also influence the secondary structure forms of HSA albumin investigated by FTIR.
Conclusion:
Both drugs tested in the concentration of human use can have an impact on the results of determination of albumin in urine which can influence clinical decision.
Related Concept Videos
Drug Distribution: Plasma Protein Binding
Drug Accumulation During Multiple Dosing: Intermittent IV Infusions
Drug Elimination by Renal Route: Tubular Secretion
Factors Affecting Renal Clearance: Drug Distribution and Drug Interactions
One important factor is the relationship between renal clearance and the apparent volume of distribution. Renal clearance tends to be inversely proportional to the apparent volume of distribution. Drugs with an extensive distribution volume or those...
Factors Affecting Protein-Drug Binding: Protein-Related Factors
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
Drug Dosing in Renal Diseases: Dose Adjustments Based on Drug Clearance and Elimination Rate Constant

