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Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Related Experiment Video

Updated: Mar 7, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
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MicroRNA Expression Profile Identifies High Grade, Non-Muscle-Invasive Bladder Tumors at Elevated Risk to Progress to

Sara M Lenherr1, Sheaumei Tsai2, Brasil Silva Neto3,4

  • 1Department of Urology, Lahey Hospital and Medical Center, Burlington, MA 01805, USA. sara.lenherr@hsc.utah.edu.

Genes
|February 21, 2017
PubMed
Summary

This study identified specific microRNAs (miRNAs) linked to bladder cancer progression. Downregulated miRNAs like miR-203a-3p and miR-205-5p indicate a higher risk of non-muscle invasive tumors progressing to muscle-invasive disease.

Keywords:
intravesical therapymicroRNAnon-muscle invasive bladder cancerprogression

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Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors
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Area of Science:

  • Urology
  • Oncology
  • Molecular Biology

Background:

  • High-grade non-muscle invasive (NMI) urothelial carcinoma can progress to muscle-invasive (MI) disease, necessitating identification of predictive biomarkers.
  • MicroRNAs (miRNAs) are key regulators of gene expression and have been implicated in cancer development and progression.

Purpose of the Study:

  • To identify differentially expressed miRNAs in high-grade NMI urothelial carcinoma that predict progression to MI disease.
  • To compare miRNA expression between non-progressive and progressive NMI lesions.
  • To investigate the association of specific miRNAs with cancer-specific survival and postoperative outcomes.

Main Methods:

  • Analysis of 89 high-grade NMI urothelial carcinoma lesions from paraffin-embedded tissues.
  • RNA extraction and microarray analysis of pooled RNA from non-progressors and progressors.
  • Quantitative reverse transcription PCR (qRT-PCR) for validation of differential miRNA expression.

Main Results:

  • Downregulation of miR-203a-3p and miR-205-5p was significantly associated with progression in NMI bladder tumors.
  • MiR-32-5p, miR-224-5p, and miR-412-3p were linked to cancer-specific survival.
  • Identified miRNAs are involved in epithelial mesenchymal transition, a process critical for tumor invasion.

Conclusions:

  • Specific miRNAs, particularly downregulated miR-203a-3p and miR-205-5p, can serve as biomarkers for predicting progression in NMI bladder cancer.
  • These findings contribute to understanding the molecular mechanisms driving bladder cancer invasion.
  • The identified miRNAs may aid in stratifying patients and guiding treatment decisions for high-grade NMI urothelial carcinoma.