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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Updated: Mar 7, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System.

Weizhong Chen1, Yifei Zhang1, Won-Sik Yeo2

  • 1School of Physical Science and Technology, ShanghaiTech University , Shanghai 201210, China.

Journal of the American Chemical Society
|February 21, 2017
PubMed
Summary

This study introduces pCasSA, a CRISPR/Cas9 system for rapid Staphylococcus aureus genome editing and gene knockdown. This tool accelerates research into drug resistance and pathogenesis for this major human pathogen.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Staphylococcus aureus is a significant human pathogen causing severe diseases with high mortality.
  • Genetic manipulation of S. aureus is crucial for understanding its physiology, drug resistance, and pathogenesis, but is traditionally time-consuming and labor-intensive.

Purpose of the Study:

  • To develop a rapid and efficient CRISPR/Cas9 system for genome editing in Staphylococcus aureus.
  • To engineer the system for gene knockdown and potential genome-wide screening applications.

Main Methods:

  • Development of the pCasSA CRISPR/Cas9 system for S. aureus.
  • Utilized Golden Gate assembly for spacer construction and Gibson assembly for repair arm assembly.
  • Engineered the system for transcription inhibition (gene knockdown).

Main Results:

  • Demonstrated rapid and efficient genome editing in S. aureus, including gene deletion, insertion, and single-base substitution.
  • Successfully engineered the pCasSA system for efficient gene knockdown.
  • The system allows for rapid plasmid construction for genome editing.

Conclusions:

  • The pCasSA CRISPR/Cas9 system significantly enhances the efficiency and speed of genetic manipulation in S. aureus.
  • This tool will accelerate the exploration of drug targets and the development of new therapeutics against S. aureus infections.