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Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
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Detecting DNA cytosine methylation using nanopore sequencing
Jared T Simpson1,2, Rachael E Workman3, P C Zuzarte1
1Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
Nature Methods
|February 21, 2017
Abstract:
In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By using synthetically methylated DNA, we were able to train a hidden Markov model to distinguish 5-mC from unmethylated cytosine. We applied our method to sequence the methylome of human DNA, without requiring special steps for library preparation.

