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Related Experiment Videos

Fowlpox virus polypeptides: sequential appearance and virion associated polypeptides.

C T Prideaux1, D B Boyle

  • 1Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T.

Archives of Virology
|January 1, 1987
PubMed
Summary

Fowlpox virus (FPV) infection of chicken embryo skin (CES) cells does not completely inhibit host protein synthesis, unlike vaccinia virus (VV). Researchers identified 21 FPV-associated polypeptides, categorized by their synthesis timing relative to viral DNA replication.

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Area of Science:

  • Virology
  • Molecular Biology
  • Cellular Biology

Background:

  • Fowlpox virus (FPV) is an avian DNA virus with significant economic impact.
  • Understanding viral protein synthesis is crucial for comprehending viral replication and pathogenesis.
  • Previous studies on vaccinia virus (VV) show host protein synthesis shutoff, but FPV's effect on host cells is less understood.

Purpose of the Study:

  • To characterize the polypeptides synthesized during fowlpox virus (FPV) infection of chicken embryo skin (CES) cells.
  • To compare FPV's effect on host cell protein synthesis with that of vaccinia virus (VV).
  • To determine the temporal synthesis patterns of FPV-induced polypeptides in relation to viral DNA replication.

Main Methods:

  • Metabolic labeling of CES cells with [35S]-methionine post-FPV infection.

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  • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to resolve and analyze synthesized polypeptides.
  • Analysis of purified FPV and VV particles using SDS-PAGE and Coomassie blue staining.

    • Main Results:

    • FPV infection of CES cells resulted in minimal inhibition of host polypeptide synthesis, contrasting with VV.
    • A total of 21 FPV-coded or induced polypeptides were identified.
    • FPV polypeptides were classified into pre-replicative and post-replicative groups, with viral DNA replication commencing between 12-16 hours post-infection.
    • Two distinct classes of post-replicative polypeptides were observed.
    • Purified FPV particles contained 57 associated polypeptides, while VV particles contained 27.

    Conclusions:

    • FPV infection exhibits a distinct pattern of host-pathogen protein interaction compared to VV.
    • The temporal regulation of FPV polypeptide synthesis is linked to viral DNA replication.
    • Detailed analysis of viral polypeptides provides insights into FPV structure and replication mechanisms.