Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TMEM132A autoimmunity in patients with suspected autoimmune cerebellar ataxia.

Journal of neuroimmunology·2026
Same author

Clinical Spectrum, Pathology, and Mechanisms of Anti-LGI4 Antibody-Positive Autoimmune Nodopathy.

Neurology(R) neuroimmunology & neuroinflammation·2025
Same author

Structural insights into heterohexameric assembly of epilepsy-related ligand-receptor complex LGI1-ADAM22.

eLife·2025
Same author

Biallelic LGI1 and ADAM23 variants cause hippocampal epileptic encephalopathy via the LGI1-ADAM22/23 pathway.

Brain : a journal of neurology·2025
Same author

Patient-derived monoclonal LGI1 autoantibodies elicit seizures, behavioral changes and brain MRI abnormalities in rodent models.

Brain, behavior, and immunity·2025
Same author

Loss of neuronal activity facilitates surface accumulation of p75NTR and cell death in avian cochlear nucleus.

Neuroscience research·2025

Related Experiment Video

Updated: Dec 27, 2025

Electrophoretic Delivery of γ-aminobutyric Acid GABA into Epileptic Focus Prevents Seizures in Mice
07:01

Electrophoretic Delivery of γ-aminobutyric Acid GABA into Epileptic Focus Prevents Seizures in Mice

Published on: May 16, 2019

9.4K

Epilepsy and synaptic proteins.

Yuko Fukata1, Masaki Fukata1

  • 1Division of Membrane Physiology, Department of Molecular and Cellular Physiology, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki 444-8787, Japan; Department of Physiological Sciences, School of Life Science, SOKENDAI, The Graduate University for Advanced Studies, Okazaki 444-8787, Japan.

Current Opinion in Neurobiology
|February 22, 2017
PubMed
Summary

Epilepsy, affecting 1-2% globally, involves synaptic protein dysfunction. New sequencing and microscopy techniques identify epilepsy-related proteins, advancing drug development and understanding synaptic transmission mechanisms.

More Related Videos

Pentylenetetrazole-Induced Kindling Mouse Model
07:06

Pentylenetetrazole-Induced Kindling Mouse Model

Published on: June 12, 2018

34.5K
Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins
09:07

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Published on: August 15, 2017

12.5K

Related Experiment Videos

Last Updated: Dec 27, 2025

Electrophoretic Delivery of γ-aminobutyric Acid GABA into Epileptic Focus Prevents Seizures in Mice
07:01

Electrophoretic Delivery of γ-aminobutyric Acid GABA into Epileptic Focus Prevents Seizures in Mice

Published on: May 16, 2019

9.4K
Pentylenetetrazole-Induced Kindling Mouse Model
07:06

Pentylenetetrazole-Induced Kindling Mouse Model

Published on: June 12, 2018

34.5K
Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins
09:07

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Published on: August 15, 2017

12.5K

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Epilepsy affects 1-2% of the global population, with causes including brain injury, toxins, and genetic mutations.
  • Synaptic proteins are crucial in epilepsy pathogenesis, with their dysfunction leading to neuronal hyperexcitability.
  • Understanding synaptic transmission is key to developing effective epilepsy treatments.

Purpose of the Study:

  • To identify novel epilepsy-related proteins using advanced genetic and proteomic techniques.
  • To elucidate the role of synaptic protein distribution in epilepsy.
  • To enhance the development of targeted anti-epileptic drugs.

Main Methods:

  • Trio exome sequencing for identifying genetic mutations in epilepsy.
  • Proteomic analysis in autoimmune synaptopathies to discover disease-related proteins.
  • Super-resolution microscopy to visualize subsynaptic protein localization.

Main Results:

  • Identification of numerous novel epilepsy-related proteins, primarily involved in synaptic transmission.
  • Revealed precise nanoscale organization of presynaptic and postsynaptic proteins at synapses.
  • Demonstrated the link between subsynaptic protein distribution and neurotransmitter release.

Conclusions:

  • Advances in sequencing and microscopy accelerate the discovery of epilepsy-related synaptic proteins.
  • Understanding synaptic protein function and localization is critical for epilepsy mechanisms.
  • This research supports the development of novel anti-epileptic therapies targeting synaptic pathways.