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Characterisation of the first KPC-2-producing Klebsiella pneumoniae ST340 from Peru

Gertrudis Horna1, Jorge Velasquez2, Nathaly Fernández2

  • 1ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain; Universidad Peruana Cayetano Heredia, Av. Honorio Delgado N.° 430, San Martín de Porras, Lima 31, Peru.

Journal of Global Antimicrobial Resistance
|February 22, 2017
PubMed
Abstract

Insights

This study identified a carbapenem-resistant Klebsiella pneumoniae isolate in Peru, carrying the KPC-2 gene within the high-risk CC258 clonal complex. Further surveillance is crucial to understand its prevalence and impact in the region.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Klebsiella pneumoniae is a significant nosocomial pathogen.
  • Carbapenem resistance, particularly due to KPC enzymes, is a growing global health concern.
  • The high-risk clonal complex 258 (CC258) is associated with the spread of carbapenem resistance.

Purpose of the Study:

  • To characterize a Klebsiella pneumoniae isolate carrying a KPC gene from a Peruvian hospital.
  • To determine the genetic features and antibiotic resistance profile of the isolate.
  • To assess the presence and potential spread of CC258 and KPC-2 in Peru.

Main Methods:

  • 16S rRNA gene sequencing for isolate identification.
  • Antibiotic resistance profiling using disk diffusion and automated methods.
  • PCR for sequence type, phylogenetic group, and beta-lactamase gene determination (including blaKPC, blaMBL, blaCTX-M, blaSHV, blaOXA, blaTEM).
  • Assessment of plasmid-encoded AmpC genes and class 1 integrons.
  • Conjugation assays to evaluate beta-lactam resistance transfer.

Main Results:

  • The isolate was confirmed as Klebsiella pneumoniae belonging to phylogenetic group KpI and sequence type ST340, a member of CC258.
  • The isolate exhibited resistance to all tested beta-lactam antibiotics.
  • A non-conjugative blaKPC-2 gene was detected, located within a non-classical genetic structure.

Conclusions:

  • This marks the first report of CC258 and a blaKPC-2 gene in Peru.
  • The findings highlight the emergence of carbapenem-resistant Klebsiella pneumoniae in Peru.
  • Intensive surveillance is recommended to ascertain the significance of CC258 and blaKPC-2 in the Peruvian healthcare setting.
Keywords:
Genetic environmentKPCPeruSouth Americaβ-Lactamase

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