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Single cell sequencing: a distinct new field.

Jian Wang1, Yuanlin Song2

  • 1Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200030, China.

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Summary

Single cell sequencing (SCS) offers high-resolution insights into biological heterogeneity. This review covers SCS technology development and its diverse clinical and research applications.

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Area of Science:

  • Genomics and Molecular Biology
  • Biotechnology

Background:

  • Biological heterogeneity is a key challenge in understanding complex biological systems.
  • Single cell sequencing (SCS) has emerged as a powerful approach to address this challenge.
  • Advancements in isolation, amplification, and sequencing technologies have propelled SCS.

Purpose of the Study:

  • To review the technological development of single cell sequencing (SCS).
  • To highlight the latest clinical and research applications of SCS across various fields.

Main Methods:

  • Review of scientific literature on SCS technologies and applications.
  • Focus on advancements in single cell isolation, genome/transcriptome amplification, and next-generation sequencing.

Main Results:

  • SCS provides high-resolution data on the genome, transcriptome, and epigenome of individual cells.
  • SCS is increasingly applied in cancer biology, oncology, immunology, microbiology, neurobiology, and prenatal diagnosis.

Conclusions:

  • SCS is a transformative technology for studying biological heterogeneity.
  • The expanding applications of SCS promise significant advancements in clinical diagnostics and biological research.