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Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity
Published on: June 14, 2024
Chitin and Stress Induced Protein Kinase Activation
Chandra Kenchappa1, Raquel Azevedo da Silva1, Simon Bressendorff1
1Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200, Copenhagen, Denmark.
Abstract:
The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.
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