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Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay
Published on: November 25, 2022
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Chitin-Triggered MAPK Activation and ROS Generation in Rice Suspension-Cultured Cells
Koji Yamaguchi1, Tsutomu Kawasaki2
1Graduate School of Agriculture, Kindai University, 3327-204 Nakamachi, Nara, 631-8505, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2017
Abstract:
Suspension-cultured cells respond sensitively to a number of stimuli including pathogen-derived molecules. Therefore, they are used in a simple, single-cell model system in order to gain insights into immune signaling pathways in rice. Here we describe protocols for studying chitin-induced MAPK activation and ROS generation in rice suspension-cultured cells.
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