Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Estrogens induce low-density lipoprotein receptor activity and decrease intracellular cholesterol in human hepatoma

C F Semenkovich1, R E Ostlund

  • 1Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.

Biochemistry
|August 11, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physiologic and genetic evidence links hemopexin to triglycerides in mice and humans.

International journal of obesity (2005)·2017
Same author

The effects of phytosterols present in natural food matrices on cholesterol metabolism and LDL-cholesterol: a controlled feeding trial.

European journal of clinical nutrition·2010
Same author

Chronic activation of AMP kinase results in NRF-1 activation and mitochondrial biogenesis.

American journal of physiology. Endocrinology and metabolism·2001
Same author

Enantiospecificity of cholesterol function in vivo.

The Journal of biological chemistry·2001
Same author

Orlistat inhibits dietary cholesterol absorption.

Obesity research·2001
Same author

Decreased hepatic accumulation and enhanced esterification of cholesterol in mice deficient in mdr1a and mdr1b P-glycoproteins.

Journal of lipid research·2001

Estrogen administration significantly boosts low-density lipoprotein (LDL) receptor activity in human liver (Hep G2) cells, potentially by reducing intracellular cholesterol. This effect was not observed in human fibroblasts.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Endocrinology

Background:

  • Estrogens are known to influence lipid metabolism.
  • Pharmacologic doses of estrogens increase hepatic low-density lipoprotein (LDL) receptor activity in animal models.

Purpose of the Study:

  • To investigate whether estrogens can regulate LDL receptor activity in human cells.
  • To determine the mechanism by which estrogens affect LDL receptor activity in human hepatoma (Hep G2) cells and fibroblasts.

Main Methods:

  • 125I-LDL binding assays
  • Ligand blotting
  • Scatchard analysis
  • Gas-liquid chromatography
  • Immunoblotting with monoclonal antibody IgG-C7

Related Experiment Videos

Main Results:

  • Estradiol increased Hep G2 cell surface LDL receptor activity by 141% but slightly reduced fibroblast receptors.
  • Estradiol induced the entire LDL receptor pathway, including internalization and degradation, in Hep G2 cells.
  • Increased LDL receptor activity in Hep G2 cells was attributed to high-affinity binding.
  • Estrogen treatment reduced free cholesterol content in Hep G2 cells but not in fibroblasts.
  • Hep G2 cells showed resistance to LDL-induced down-regulation of LDL receptor activity compared to fibroblasts.

Conclusions:

  • Estrogens can up-regulate LDL receptor activity in human hepatoma cells, possibly indirectly by lowering intracellular cholesterol.
  • The LDL receptor in Hep G2 cells is biochemically similar to the extrahepatic LDL receptor found in fibroblasts.
  • Hep G2 cells exhibit distinct regulation of LDL receptor activity compared to normal human fibroblasts.