A 4,5-quinolimide-based fluorescent sensor for the turn-on detection of Cd²⁺ with live-cell imaging

A 4,5-quinolimide derivative, BNA, bearing the amide-DPA receptor, was synthesized as a turn-on fluorescent sensor for Cd²⁺. Under physiological conditions, BNA could distinguish Cd²⁺ from Zn²⁺, showing turn-on fluorescence behaviour and an increased fluorescence lifetime. BNA and Cd²⁺ formed a 1 : 1 stoichiometric complex, and the detection limit was measured to be as low as 11 nM. Furthermore, BNA was utilized for fluorescence imaging of Cd²⁺ in live cells. To the best of our knowledge, it is the first 4,5-quinolimide-based sensor for the detection of metal ions.