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Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Published on: December 20, 2019

Rab7A regulates tau secretion.

Lilia Rodriguez^1,2,3, Nguyen-Vi Mohamed^1,2,3, Alexandre Desjardins^1,2,3

¹Research Center of the University of Montreal Hospital (CRCHUM), Montréal, Québec, Canada.

Journal of Neurochemistry

|February 22, 2017

Summary

This summary is machine-generated.

Rab7A regulates the secretion of TAU protein, a key factor in Alzheimer's disease (AD) pathology. This finding suggests that increased RAB7A in AD brains may worsen TAU spread and disease progression.

Keywords:

Alzheimer's disease Rab7A secretion tau protein

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Area of Science:

Neuroscience
Cell Biology
Biochemistry

Background:

TAU protein is implicated in Alzheimer's disease (AD) and can spread extracellularly.
Rab7A, a small GTPase, is involved in intracellular trafficking and is upregulated in AD.

Purpose of the Study:

To investigate the role of Rab7A in the secretion of TAU protein.
To test the hypothesis that Rab7A regulates TAU secretion.

Main Methods:

Utilized primary cortical neurons and HeLa cells overexpressing human TAU.
Assessed TAU secretion following Rab7A deletion or manipulation of its activity (dominant-negative and constitutively active forms).
Examined co-localization of TAU and Rab7-positive structures.

Main Results:

Rab7A deletion significantly decreased TAU secretion.
Overexpression of dominant-negative Rab7A reduced TAU secretion, while constitutively active Rab7A increased it.
Partial co-localization of TAU and Rab7 suggests involvement of late endosomes in secretion.

Conclusions:

Rab7A plays a regulatory role in TAU secretion.
Upregulation of RAB7A in AD may contribute to extracellular TAU accumulation and pathology propagation.