Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrasound modified flaxseed protein-rice starch composite system for texture-modified soft gels: structural, functional, and swallowing properties.

Ultrasonics sonochemistry·2026
Same author

Sertoli cells, but not testicular endothelial cells, contribute to optimized culture of spermatogonia in pigs.

Reproduction (Cambridge, England)·2026
Same author

Hexavalent chromium induces death of porcine spermatogonial stem cells via promoting mitophagy and ferroptosis.

Environmental toxicology and pharmacology·2026
Same author

Light-Gated Thermal Domains in Nano-Lanterns: Confined Heat Hotspots Sparks Electron Localization for Water Purification.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Antibiotic Residues in Muscle Tissues of Lueyang Black-Bone Chickens Under Free-Range Mountainous Conditions and Their Association with Gut Microbiota.

Microorganisms·2025
Same author

Imiquimod (R837), a TLR7-Specific Agonist, Regulates Boar Sperm Motility via PI3K/GSK3α/β/Hexokinase Pathway.

Biology·2025

Related Experiment Video

Updated: Mar 7, 2026

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
07:02

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

13.5K

Trehalose improves rabbit sperm quality during cryopreservation.

Zhendong Zhu1, Xiaoteng Fan1, Yang Pan1

  • 1College of Animal Science and Technology, Northwest A&F University, Shaanxi, 712100, China.

Cryobiology
|February 23, 2017
PubMed
Summary
This summary is machine-generated.

Adding trehalose to freezing extenders improves rabbit sperm survival after cryopreservation. This method enhances sperm motility and integrity by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels.

Keywords:
AntioxidantsCryopreservationRabbitSemenTrehalose

More Related Videos

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
11:01

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

32.2K
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
13:32

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Published on: March 4, 2018

8.9K

Related Experiment Videos

Last Updated: Mar 7, 2026

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
07:02

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

13.5K
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
11:01

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

32.2K
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
13:32

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Published on: March 4, 2018

8.9K

Area of Science:

  • Reproductive Biology
  • Cryobiology
  • Biochemistry

Background:

  • Cryopreservation of spermatozoa often leads to damage from high levels of reactive oxygen species (ROS).
  • Maintaining sperm function and viability post-thaw is crucial for artificial insemination and breeding programs.

Purpose of the Study:

  • To evaluate the protective effects of trehalose supplementation in freezing extenders on rabbit spermatozoa.
  • To investigate the impact of trehalose on sperm parameters and oxidative stress markers.

Main Methods:

  • Rabbit semen was diluted in Tris-citrate-glucose extender with varying concentrations of trehalose.
  • Post-thaw sperm parameters including motility, acrosome integrity, membrane integrity, and mitochondrial membrane potential were assessed.
  • Antioxidant enzyme activities (catalase, superoxide dismutase), total antioxidant capacity (T-AOC), ROS, and malondialdehyde (MDA) levels were measured.

Main Results:

  • Supplementation with 100 mM trehalose significantly improved post-thaw sperm motility, acrosome integrity, membrane integrity, and mitochondrial membrane potential.
  • Trehalose enhanced the activities of catalase (CAT), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC).
  • A significant decrease in reactive oxygen species (ROS) levels and malondialdehyde (MDA) content was observed in trehalose-supplemented extenders.

Conclusions:

  • Supplementing freezing extenders with 100 mM trehalose effectively mitigates cryopreservation-induced damage in rabbit spermatozoa.
  • Trehalose enhances sperm viability and function by reducing oxidative stress and improving antioxidant capacity.
  • Trehalose supplementation offers a promising strategy for improving success rates in the rabbit breeding industry.