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Characterization of calpains and calpastatins from hamster skeletal muscle
P Johnson1, J L Guindon-Hammer
1Department of Chemistry, Ohio University, Athens 45701.
Abstract:
1. Hamster skeletal muscle contains a wide-specificity calpain which was found to be a calpain II type and which is composed of a single Mr 80,000 polypeptide. 2. The muscle also contains a calpain I type enzyme which is specific for desmin degradation, and this enzyme consists of a single subunit of Mr 67,000. 3. Three calpastatins were detected in the tissue, one of which inhibited both calpains, whereas the other two appeared to be specific for the desmin-specific calpain. These calpastatins possessed the same inhibition properties when assayed with chicken gizzard calpains.