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Related Experiment Video

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A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models.

N Bono1,2, S Meghezi2, M Soncini1

  • 1μBS Lab, Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, P.zza Leonardo da Vinci 32, 20133, Milan, Italy.

Annals of Biomedical Engineering
|February 23, 2017
PubMed
Summary

This study introduces a novel dual-mode bioreactor for engineering vascular tissues. Cyclic strain stimulation enhanced collagen matrix compaction and smooth muscle cell function, improving mechanical strength compared to static cultures.

Keywords:
BioreactorCollagenCyclic strainMechanical propertiesSmooth muscle cellsVascular tissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Biotechnology

Background:

  • Vascular tissue engineering aims to create clinical therapies and research tools.
  • Collagen-based tubular constructs are promising for vascular applications.
  • Bioreactors are crucial for in vitro tissue development and study.

Purpose of the Study:

  • To design and characterize a novel dual-mode bioreactor for fabricating and stimulating collagen-based vascular constructs.
  • To investigate the effects of cyclic strain on cell-matrix interactions and construct properties.
  • To compare cell behavior under cyclic stimulation versus static culture.

Main Methods:

  • Fabrication of collagen-gel constructs with smooth muscle cells (SMCs) within a dual-mode bioreactor.
  • Application of 10% cyclic strain at 0.5 Hz for 5 days in culture mode.
  • Assessment of matrix rearrangement, biomechanical properties, cell localization, and phenotypic markers.

Main Results:

  • Cyclic stimulation induced cell-driven collagen matrix compaction, enhancing mechanical strength.
  • Strained constructs showed uniform cell distribution and maintained SMC contractile phenotype.
  • Static culture led to cell polarization and reduced cell density.

Conclusions:

  • The dual-mode bioreactor is effective for fabricating and maturing collagen-based vascular constructs.
  • Mechanical conditioning via cyclic strain improves construct biomechanics and cell behavior.
  • This technology aids in studying cell and tissue responses to controlled mechanical environments.