Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glucose Transporters01:27

Glucose Transporters

27.8K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
27.8K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

1.1K
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
1.1K
Membrane Proteins01:30

Membrane Proteins

31.1K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
31.1K
Glucose Homeostasis: Regulation of Blood Glucose01:02

Glucose Homeostasis: Regulation of Blood Glucose

4.9K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
4.9K
Secondary Active Transport01:55

Secondary Active Transport

139.4K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
139.4K
Secondary Active Transport01:32

Secondary Active Transport

12.3K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent trends in dual-acting hybrid antibiotics and combination therapies against Gram-negative pathogens.

Journal of microbiology (Seoul, Korea)·2026
Same author

Sex-Dependent Microbial and Host Profiles Following Fecal Microbiota and <i>Bifidobacterium longum</i> Treatment in Stress-Induced Gut Dysbiosis.

Gut and liver·2026
Same author

Identification of Genetic and Environmental Factors Suppressing the Lethality and Antibiotic Susceptibility Mediated by Depletion of LptD, a Lipopolysaccharide Transport Protein.

Journal of microbiology and biotechnology·2025
Same author

Inhibition of cardiolipin biosynthesis partially suppresses the sensitivity of an Escherichia coli mutant lacking OmpC to envelope stress.

Journal of microbiology (Seoul, Korea)·2025
Same author

Coevolutionary signals in multiple sequence alignments improve virulence factor prediction with an MSA Transformer.

Scientific reports·2025
Same author

Structural basis of the catalytic and allosteric mechanism of bacterial acetyltransferase PatZ.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Mar 7, 2026

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
06:21

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

Published on: April 7, 2023

2.2K

The general PTS component HPr determines the preference for glucose over mannitol.

Mangyu Choe1, Young-Ha Park1, Chang-Ro Lee2

  • 1School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 00826, Korea.

Scientific Reports
|February 23, 2017
PubMed
Summary

In E. coli, glucose preference over mannitol relies on the HPr protein, not EIIA^Glc. Dephosphorylated HPr binds MtlR, inhibiting mannitol operon expression and sugar utilization.

More Related Videos

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model
09:44

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Published on: March 5, 2022

3.5K
Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
11:10

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Published on: November 27, 2013

14.3K

Related Experiment Videos

Last Updated: Mar 7, 2026

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
06:21

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

Published on: April 7, 2023

2.2K
Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model
09:44

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Published on: March 5, 2022

3.5K
Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
11:10

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Published on: November 27, 2013

14.3K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Preferential carbon source utilization is common in bacteria.
  • The phosphoenolpyruvate:sugar phosphotransferase system (PTS) mediates sugar uptake.
  • Glucose preference over non-PTS sugars is linked to enzyme IIA^Glc (EIIA^Glc) phosphorylation state.

Purpose of the Study:

  • To elucidate the mechanism of preference between PTS sugars in Gram-negative bacteria.
  • To investigate the role of HPr and EIIA^Glc in glucose versus mannitol utilization in E. coli.

Main Methods:

  • Genetic analysis of PTS components in E. coli.
  • Investigating protein-protein interactions between HPr and MtlR.
  • Assessing the impact of HPr phosphorylation state on mannitol operon expression.

Main Results:

  • Glucose preference over mannitol in E. coli depends on the general PTS component HPr, not EIIA^Glc.
  • Dephosphorylated HPr accumulates during glucose transport.
  • HPr interacts with MtlR, enhancing its repressor activity and inhibiting mannitol utilization.

Conclusions:

  • The mechanism for PTS sugar preference involves HPr modulating MtlR activity.
  • This study reveals a novel regulatory role for HPr in carbon catabolite repression.
  • Understanding PTS sugar preference is crucial for microbial metabolism and biotechnology.