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Microfluidic Device for Studying Controllable Hydrodynamic Flow Induced Cellular Responses.

Chunhong Zheng1, Xiannian Zhang1, Chunmei Li1

  • 1Beijing Advanced Innovation Center for Genomics (ICG), Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, College of Engineering, and Peking-Tsinghua Center for Life Sciences, Peking University , Beijing 100871, China.

Analytical Chemistry
|February 23, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a microfluidic device to study how hydrodynamic flow affects endothelial cells. This technology reveals how flow patterns influence cell behavior and gene expression, aiding atherosclerosis research.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Fluid Dynamics

Background:

  • Hydrodynamic flow is critical for cellular functions and linked to diseases like atherosclerosis.
  • Blood flow patterns significantly impact endothelial cells' gene expression and functions.
  • Existing tools for studying flow-induced cellular responses are often limited in precision and portability.

Purpose of the Study:

  • To develop an integrated microfluidic device for precise control of flow patterns on endothelial cells.
  • To investigate the effects of uni- or bidirectional flow on human umbilical vein endothelial cells.
  • To correlate cell phenotypic changes with gene expression alterations under shear stress.

Main Methods:

  • Development of an integrated microfluidic device for controlled hydrodynamic flow.
  • Culturing human umbilical vein endothelial cells on-chip.
  • Monitoring cell morphology.
  • Utilizing small-input RNA sequencing (RNA-seq) for transcriptome profiling.

Main Results:

  • The microfluidic device precisely generated different flow patterns.
  • Uni- or bidirectional flow induced distinct changes in endothelial cell morphology and transcriptome profiles.
  • The study provided new transcriptomic data on endothelial cell responses to shear stimulus.

Conclusions:

  • The developed microfluidic device offers a powerful tool for studying shear-induced endothelial cell functions.
  • This technology enhances understanding of the relationship between cell phenotype and gene expression changes.
  • The findings contribute to understanding hemodynamics in relation to endothelial cell biology and disease.