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Reversible interconversion between primitive endoderm- and parietal endoderm-like F9 cells demonstrated by mRNAs
K Miki1, E Sugimoto, Y Kitagawa
1Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Aichi.
Journal of Biochemistry
|August 1, 1987
Summary
Retinoic acid-treated F9 cells differentiate into parietal endoderm-like cells, a process reversible by removing dibutyryl cAMP. This study identifies key mRNAs, including laminin B, involved in early mouse embryo morphogenesis.
Area of Science:
- Developmental Biology
- Cell Differentiation
- Molecular Biology
Background:
- F9 cells serve as a model for early mouse embryo development.
- Parietal endoderm differentiation is crucial for embryonic morphogenesis.
- Understanding differentiation signals is key to developmental studies.
Purpose of the Study:
- To investigate the differentiation of F9 cells into parietal endoderm-like cells.
- To identify and characterize specific mRNAs expressed during this differentiation process.
- To explore the reversibility of parietal endoderm differentiation.
Main Methods:
- Retinoic acid and dibutyryl cAMP treatment of F9 cells.
- Selection and characterization of cDNA clones specific to parietal endoderm.
- Northern hybridization and dot hybridization for mRNA analysis.
- In vitro translation to identify protein products.
Main Results:
- Identified 6 cDNA clones specific to parietal endoderm-like F9 cells.
- Demonstrated reversible mRNA expression dependent on dibutyryl cAMP.
- Isolated clone pLAM, corresponding to laminin B subunit mRNA.
- Showed over 100-fold stimulation of laminin B expression during differentiation.
Conclusions:
- F9 cell differentiation into parietal endoderm is reversible upon removal of signaling molecules.
- Laminin B expression is significantly upregulated during F9 cell differentiation.
- This model provides insights into the regulation of early embryonic morphogenesis.