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Related Experiment Videos

Three differentially expressed Na,K-ATPase alpha subunit isoforms: structural and functional implications.

V L Herrera1, J R Emanuel, N Ruiz-Opazo

  • 1Laboratory of Molecular and Cellular Cardiology, Howard Hughes Medical Institute, Boston, Massachusetts.

The Journal of Cell Biology
|October 1, 1987
PubMed
Summary
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Researchers identified three sodium-potassium ATPase (Na,K-ATPase) alpha subunit isoforms in rats. These isoforms show tissue-specific and developmental expression, suggesting functional diversity within this essential ion pump.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • The sodium-potassium ATPase (Na,K-ATPase) is a crucial enzyme for maintaining cellular ion gradients.
  • Understanding the diversity of its alpha subunit isoforms is key to comprehending its varied physiological roles.

Purpose of the Study:

  • To characterize the cDNAs encoding three Na,K-ATPase alpha subunit isoforms in the ouabain-resistant rat.
  • To investigate the tissue-specific and developmental expression patterns of these isoforms.

Main Methods:

  • cDNA characterization
  • Northern blot analysis
  • S1-nuclease mapping

Main Results:

  • Identified three distinct Na,K-ATPase alpha subunit isoforms in rats.

Related Experiment Videos

  • Demonstrated tissue-specific and developmentally regulated expression of alpha subunit mRNAs.
  • Observed differential expression patterns for alpha 1, alpha 2, and alpha 3 isoforms across various tissues and developmental stages.
  • Deduced highly conserved amino acid sequences among isoforms, yet distinct codon usage and genomic hybridization patterns suggest a multigene family.

    • Conclusions:

    • Rat Na,K-ATPase alpha subunits are encoded by a multigene family.
    • Isoform diversity in Na,K-ATPase alpha subunits likely contributes to functional diversity.
    • Structural analysis predicts a polytopic protein with seven membrane-spanning regions.