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Studies on the attenuation of the Sabin type 3 oral polio vaccine
J W Almond1, G D Westrop, D M Evans
1Department of Microbiology, University of Reading, U.K.
Journal of Virological Methods
|August 1, 1987
Abstract:
The genetic basis of attenuation of the poliovirus type 3 vaccine strain P3/Leon 12a1b has been investigated by comparing the nucleotide sequence of this strain with that of its neurovirulent progenitor P3/Leon/37 and by constructing recombinants between these two viruses using infectious cDNAs. Preliminary results suggest that attenuation is caused by just two point mutations, one occurring in the 5' non-coding region and the other causing an amino acid change in coat protein VP3.