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Capsid Phosphorylation State and Hepadnavirus Virion Secretion
Xiaojun Ning1, Suresh H Basagoudanavar1, Kuancheng Liu1
1Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA.
Journal of Virology
|February 24, 2017
Summary
Hepatitis B virus (HBV) core protein
Area of Science:
- Virology
- Molecular Biology
- Hepatitis B Virus Research
Background:
- The C-terminal domain (CTD) of hepadnavirus core protein is crucial for viral replication, with phosphorylation playing key roles.
- CTD phosphorylation facilitates RNA packaging and early DNA synthesis, while dephosphorylation aids later DNA synthesis and nucleocapsid stabilization in duck hepatitis B virus (DHBV).
- The specific role of CTD phosphorylation in hepatitis B virus (HBV) virion secretion remained unclear.
Purpose of the Study:
- To investigate the role of CTD phosphorylation in HBV virion secretion.
- To compare CTD phosphorylation states in complete (DNA-containing) versus empty (genome-free) HBV virions.
- To determine if CTD phosphorylation or dephosphorylation is essential for the secretion of both DNA-containing and empty HBV virions.
Main Methods:
- Analysis of CTD phosphorylation status in complete and empty HBV virions.
- Site-directed mutagenesis of the CTD to mimic phosphorylation or dephosphorylation.
- Assessment of virion secretion following CTD modifications in HBV and DHBV.
Main Results:
- CTD is dephosphorylated in complete HBV virions associated with maturation and secretion, similar to DHBV.
- CTD is phosphorylated in empty HBV virions.
- Mutational analyses showed that neither CTD phosphorylation nor dephosphorylation is essential for the secretion of DNA-containing or empty HBV virions.
Conclusions:
- CTD dephosphorylation is associated with nucleocapsid maturation and HBV DNA synthesis but not essential for virion secretion.
- The phosphorylation state of the CTD does not appear to regulate the secretion of either DNA-containing or empty HBV virions.
- CTD phosphorylation dynamics play multifaceted roles in the HBV life cycle, independent of virion secretion.
Keywords:
C-terminal domaincapsid proteindephosphorylationduck hepatitis B virushepadnavirushepatitis B virusmaturationphosphorylationvirion secretionMore Related Videos
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