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Related Experiment Video

Updated: Mar 7, 2026

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
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Endothelial Progenitor Cells: Procedure for Cell Isolation and Applications.

Venkata N S Garikipati1, Raj Kishore2,3

  • 1Center for Translational Medicine, Lewis Katz School of Medicine, Temple University, MERB-953, 3500 N Broad Street, Philadelphia, PA, 19140, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 24, 2017
PubMed
Summary

Bone marrow endothelial progenitor cells (EPCs) show promise for healing ischemic tissues. This chapter details protocols for isolating, characterizing, and culturing EPCs for research and clinical applications.

Keywords:
AngiogenesisBone marrowEndothelialNeo-vascularizationProgenitor cells

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Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Vascular Biology

Background:

  • Endothelial progenitor cells (EPCs) from bone marrow are crucial for neovascularization and mitigating ischemic damage.
  • EPC-based therapies have shown modest but promising results in clinical trials for ischemic conditions.
  • Lack of a unique marker complicates EPC isolation and characterization, leading to variability in research.

Purpose of the Study:

  • To provide standardized, step-by-step protocols for isolating and characterizing bone marrow EPCs.
  • To outline optimal culture conditions for EPCs.
  • To discuss the potential applications of EPCs in basic and clinical research.

Main Methods:

  • Detailed protocols for the isolation of EPCs from bone marrow.
  • Methods for characterizing EPCs, addressing the lack of a unique marker.
  • Guidelines for maintaining EPCs in culture.

Main Results:

  • Established reproducible methods for obtaining functional EPCs.
  • Characterization techniques to ensure purity and identify progenitor populations.
  • Defined culture conditions supporting EPC survival and proliferation.

Conclusions:

  • Standardized EPC isolation and characterization are essential for reliable research and clinical translation.
  • Defined protocols will facilitate further investigation into EPC therapeutic potential.
  • This chapter provides a valuable resource for researchers and clinicians working with EPCs.