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CSFV proliferation is associated with GBF1 and Rab2

Wulong Liang1, Minping Zheng, Changlei Bao

  • 1College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi 712100, People's Republic of China.

Journal of Biosciences
|February 24, 2017
PubMed

Insights

Classical swine fever virus (CSFV) uses the Golgi apparatus for proliferation. Inhibiting Golgi function with chemicals or gene silencing significantly reduced CSFV replication, while enhancing specific Golgi proteins promoted it.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Viruses exploit cellular machinery, including the Golgi apparatus, for replication.
  • Understanding host-pathogen interactions at the Golgi is crucial for antiviral strategies.

Purpose of the Study:

  • To investigate the role of Golgi apparatus function in Classical swine fever virus (CSFV) proliferation.
  • To determine the involvement of GBF1 and Rab2 GTPases in CSFV multiplication.

Main Methods:

  • Disturbance of Golgi function using chemical inhibitors: brefeldin A (BFA) and golgicide A (GCA).
  • Genetic manipulation of Golgi-specific proteins GBF1 and Rab2 using shRNA and lentiviral overexpression systems.
  • Assessment of CSFV proliferation, RNA replication, and viral particle generation.

Main Results:

  • BFA and GCA treatments significantly inhibited CSFV RNA replication and viral particle formation.
  • Silencing GBF1 and Rab2 expression using shRNA retarded CSFV multiplication dynamics.
  • Overexpression of GBF1 and Rab2 promoted CSFV proliferation.

Conclusions:

  • Golgi apparatus function is essential for efficient CSFV multiplication.
  • GBF1 and Rab2 play a significant role in supporting CSFV proliferation.
  • Further research into Golgi-resident proteins is needed to fully elucidate CSFV replication mechanisms.

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