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Related Experiment Videos

Histamine-stimulated phosphoinositide hydrolysis in developing rat brain.

E Claro1, A García, F Picatoste

  • 1Instituto de Biologia Fundamental Vicent Villar Palasi, Universidad Autónoma de Barcelona, Spain.

Molecular Pharmacology
|September 1, 1987
PubMed
Summary

Histamine stimulates phosphoinositide breakdown in developing rat brains, with a response involving non-H1 receptor mechanisms early on. This suggests a developmental shift in histamine signaling pathways.

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Developmental Biology

Background:

  • Histamine signaling is crucial for brain function.
  • Phosphoinositide hydrolysis is a key signaling pathway.
  • Understanding developmental changes in neurotransmitter systems is vital.

Purpose of the Study:

  • To investigate the developmental pattern of histamine-stimulated phosphoinositide hydrolysis in the rat brain cortex.
  • To characterize the receptor mechanisms involved in this process during development.

Main Methods:

  • Measurement of [3H]inositol monophosphate accumulation in lithium-treated rat cortical slices.
  • Analysis of concentration-effect curves using nonlinear regression.
  • Receptor binding assays with [3H]mepyramine.

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  • Pharmacological characterization using receptor agonists and antagonists.

    • Main Results:

    • Histamine stimulated phosphoinositide hydrolysis from early postnatal stages (day 3).
    • A biphasic response (high and low potency components) was observed in young rats, contrasting with a single component in adults.
    • H1 receptor binding increased significantly with age, but the functional response to histamine showed a different developmental trajectory.
    • A portion of the histamine response in young rats was independent of H1 receptor activation.

    Conclusions:

    • Histamine activates phosphoinositide hydrolysis via both H1 receptor-dependent and -independent pathways in the developing rat brain.
    • The contribution of the non-H1 receptor pathway diminishes with age.
    • This suggests a developmental maturation of histamine signaling, potentially involving H1 receptor subsensitivity.