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Related Experiment Videos

Interaction between CD4 and class II MHC molecules mediates cell adhesion.

C Doyle1, J L Strominger

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Nature
|November 19, 1987
PubMed
Summary

The CD4 glycoprotein directly binds to class II major histocompatibility complex (MHC) antigens. This interaction functions as a cell surface adhesion molecule, independent of T-cell receptor activity.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • The CD4 glycoprotein is crucial for T-helper and cytotoxic lymphocytes.
  • Antibody studies suggest CD4 enhances effector-target cell interactions by binding to class II MHC antigens.
  • Previous research lacked direct evidence of CD4 and class II molecule interaction.

Purpose of the Study:

  • To investigate the direct interaction between the CD4 glycoprotein and class II major histocompatibility complex (MHC) molecules.
  • To determine if CD4 can function as a cell surface adhesion molecule through direct binding to class II antigens.

Main Methods:

  • Expression of human CD4 glycoprotein in CV1 cells using a simian virus 40 (SV40) vector.
  • Incubation of CD4-expressing CV1 cells with human B cells expressing MHC class II molecules.

Related Experiment Videos

  • Assessment of binding and inhibition using anti-class II and anti-CD4 antibodies.

    • Main Results:

    • CD4-expressing CV1 cells exhibited tight binding to B cells bearing class II MHC molecules.
    • Mutant B cells lacking class II molecules did not bind to CD4-expressing cells.
    • The binding was specifically inhibited by antibodies targeting CD4 and class II molecules.

    Conclusions:

    • The CD4 glycoprotein directly interacts with class II MHC antigens.
    • This direct interaction enables CD4 to function as a cell surface adhesion molecule.
    • CD4-mediated adhesion is independent of T-cell receptor-antigen interactions.