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The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung

Hua Zhang1, Yaqiong Xiong2, Rui Xia3

  • 11 Department of Joint Trauma, Junan County People's Hospital, Linyi, People's Republic of China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|February 25, 2017
PubMed

Insights

Pseudogenes, once thought functionless, are transcribed in cancers. This study identifies SFTA1P, a pseudogene-derived long noncoding RNA, as a tumor suppressor in lung adenocarcinoma, potentially aiding diagnosis and therapy.

Area of Science:

  • Genomics
  • Cancer Biology
  • Noncoding RNA Research

Background:

  • Pseudogenes were historically considered non-functional genomic relics.
  • Emerging evidence highlights pseudogene transcription in human cancers, influencing disease development.
  • Pseudogene transcripts, including long noncoding RNAs (lncRNAs), play roles in cancer progression.

Purpose of the Study:

  • To identify and characterize novel pseudogene-derived lncRNAs in non-small cell lung cancer (NSCLC).
  • To investigate the functional role and clinical significance of the identified pseudogene SFTA1P in NSCLC, particularly lung adenocarcinoma.
  • To explore the molecular pathways associated with SFTA1P in lung adenocarcinoma.

Main Methods:

  • Analysis of Gene Expression Omnibus (GEO) microarray data for NSCLC.
  • Differential expression analysis of SFTA1P in NSCLC tissues versus normal tissues.
  • Gain-of-function studies (in vitro and in vivo) to assess SFTA1P's tumor suppressor activity.
  • Correlation analysis of SFTA1P expression with patient survival data.
  • Investigation of SFTA1P-associated genes using The Cancer Genome Atlas (TCGA) RNA sequencing data.

Main Results:

  • SFTA1P was identified as a novel pseudogene-derived lncRNA.
  • SFTA1P expression was significantly downregulated in NSCLC tissues.
  • Decreased SFTA1P expression correlated with poorer survival in lung adenocarcinoma patients, but not lung squamous cell carcinoma.
  • Functional studies confirmed SFTA1P's tumor suppressor role in NSCLC by inhibiting growth, migration, and invasion.
  • Potential molecular pathways influenced by SFTA1P in lung adenocarcinoma were elucidated.

Conclusions:

  • Pseudogene-derived lncRNA SFTA1P functions as a tumor suppressor in human lung adenocarcinoma.
  • SFTA1P represents a novel potential biomarker for lung adenocarcinoma diagnosis.
  • SFTA1P may offer a new therapeutic target for lung adenocarcinoma treatment.
Keywords:
PseudogeneSFTA1Plong noncoding RNAlung adenocarcinomatumor suppressor

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