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Comprehensive analysis of long non-coding RNAs highlights their spatio-temporal expression patterns and evolutional
Zhonglin Tang1,2, Yang Wu3, Yalan Yang1,2
1State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Scientific Reports
|February 25, 2017
Summary
This study identifies 10,813 long non-coding RNAs (lncRNAs) in miniature pigs, revealing conserved functions and expression patterns with humans and mice. These lncRNAs are crucial for mammalian organ development and physiology.
Area of Science:
- Genomics
- Molecular Biology
- Developmental Biology
Background:
- Long non-coding RNAs (lncRNAs) show conserved expression and function despite sequence divergence.
- Comprehensive analysis of lncRNAs across mammalian tissues and developmental stages is lacking.
Purpose of the Study:
- To systematically investigate lncRNAs in the Guizhou miniature pig (Sus scrofa), a biomedical model.
- To characterize lncRNA conservation, expression patterns, and potential functions in mammalian development.
Main Methods:
- RNA sequencing across nine organs and three developmental skeletal muscle stages.
- Development of a filtering pipeline to identify and analyze lncRNAs.
- Genome alignment for conservation analysis and weighted co-expression network analysis.
Main Results:
- Identified 10,813 lncRNAs, including 9,075 novel ones.
- 57% of pig lncRNAs showed homology to human and mouse lncRNAs.
- 5,455 lncRNAs displayed high spatio-temporal specificity, with conserved lncRNAs enriched in gonads.
- Conserved lncRNAs implicated in postnatal muscle development.
Conclusions:
- Pig lncRNAs share structural and expression similarities with human and mouse lncRNAs.
- lncRNAs play significant roles in mammalian organ physiology and development.
- This study provides a valuable resource for animal evolution, breeding, and biomedical research.
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