Related Experiment Video
Updated: Mar 7, 2026

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
Published on: April 17, 2016
Activation of secondary cell wall biosynthesis by miR319-targeted TCP4 transcription factor
Xudong Sun1,2,3, Chongde Wang4, Nan Xiang1,2,3
1Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
Abstract:
The overexpression of miR319 in plants results in delayed senescence, and high levels of miR319-targeted TCP4 transcription factor cause premature onset of this process. However, the underlying mechanisms of this pathway remain elusive. Here, we found that miR319 overexpression results in a decrease in TCP4 abundance and secondary cell wall formation in the stem. Conversely, constitutive expression of miR319-resistant TCP4 promotes secondary cell wall formation, indicating that miR319-mediated TCP4 controls secondary cell wall formation during development. Further analysis revealed that TCP4 might directly bind the promoter of VND7 to activate its expression, which triggers the expression of a VND7 transcriptional network associated with secondary cell wall biosynthesis and programmed cell death and accelerates vessel formation. In addition, the development process gradually increased TCP4 expression. These results suggest that miR319 and its target TCP4 can act as switches that turn on secondary cell wall synthesis and programmed cell death.
More Related Videos
05:143-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens
Published on: February 10, 2023
11:50Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
Published on: October 1, 2015
Related Concept Videos
Role of Microtubules in Cell Wall Deposition
Plant Cell Wall
Plant Cell Wall
Gene Regulation During Sporulation
Bacterial Cell Wall
Stringent Response in E. coli