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Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy
Fei Ren1,2,3, Long Chen2, Shuangli Xiong3
1School of Life Science and Technology, Southwest University of Science and Technology, Mianyang, Sichuan, China.
Plos One
|February 25, 2017
Summary
Optimizing fermentation conditions, including pH and nutrient feeding, significantly boosted acarbose production by Streptomyces M37. A two-stage strategy increased acarbose yield by 85.7%, linked to key enzyme activity.
Area of Science:
- Microbiology
- Biotechnology
- Biochemistry
Background:
- Acarbose is an important antidiabetic drug.
- Optimizing fermentation processes is crucial for increasing the yield of microbial products.
- Streptomyces M37 is a known producer of acarbose.
Purpose of the Study:
- To investigate the impact of pH on Streptomyces M37 growth and acarbose biosynthesis.
- To develop an optimized fermentation strategy for enhanced acarbose production.
- To elucidate the biochemical basis for improved acarbose yield.
Main Methods:
- Cultivation of Streptomyces M37 under varying pH conditions.
- Implementation of a two-stage fermentation strategy with controlled pH and nutrient feeding (glucose and maltose).
- Measurement of acarbose titer and enzyme activities (glutamate dehydrogenase, glucose 6-phosphate dehydrogenase).
Main Results:
- Low pH favored Streptomyces M37 growth, while high pH enhanced acarbose synthesis.
- Feeding glucose and maltose after 72 hours significantly promoted acarbose production.
- The optimized two-stage fermentation strategy resulted in an 85.7% increase in acarbose titer (6210 mg/L).
Conclusions:
- A two-stage fermentation strategy, involving initial growth at pH 7.0 and subsequent production at pH 8.0 with nutrient feeding, is effective for enhancing acarbose production.
- Increased acarbose production is associated with elevated activity of glutamate dehydrogenase and glucose 6-phosphate dehydrogenase.
