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Modulation of autophagy in exJSRV-env-transfected cells through the Akt/mTOR and MAPK signaling pathway

Xiaolin Sun1, Fangyuan Du1, Shuying Liu1

  • 1College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot 010018, China.

Biochemical and Biophysical Research Communications
|February 26, 2017
PubMed

Insights

Jaagsiekte sheep retrovirus envelope (Env) oncoprotein inhibits autophagy via Akt/mTOR and MAPK pathways. This study clarifies JSRV Env

Area of Science:

  • Oncology
  • Virology
  • Cell Biology

Background:

  • Jaagsiekte sheep retrovirus (JSRV) envelope (Env) is an oncoprotein implicated in ovine pulmonary adenocarcinoma (OPA).
  • The role of autophagy in JSRV Env-induced carcinogenesis is not fully understood.
  • Autophagy modulation is a potential target in cancer therapy.

Purpose of the Study:

  • To investigate the modulation of autophagy in JSRV Env-transfected cells.
  • To elucidate the involvement of Akt/mTOR and MAPK signaling pathways in JSRV Env-mediated autophagy.
  • To determine the mechanism by which JSRV Env affects autophagy.

Main Methods:

  • Immunohistochemical and microscopic analysis of OPA lung tissues and JSRV Env-transfected NIH 3T3 cells.
  • Assessment of autophagy markers (Beclin1, LC3 II/I) and signaling pathway proteins (p-Akt, p-mTOR, p-MEK1/2, p-ERK1/2, p-38, p-JNK).
  • Treatment of transfected cells with specific inhibitors for mTOR, ERK1/2, p38, and JNK pathways.

Main Results:

  • Activated Akt/mTOR and MAPK pathways were observed in OPA lung tissues and JSRV Env-transfected cells.
  • Autophagy was inhibited in OPA lung tissues and JSRV Env-transfected cells, indicated by decreased Beclin1 and LC3 II/I.
  • Inhibition of autophagy markers was reversed by treatment with mTOR, ERK1/2, p38, and JNK inhibitors.

Conclusions:

  • JSRV Env decreases autophagy in transfected cells through the activation of Akt/mTOR and MAPK signaling pathways.
  • The JNK and p38 pathways are particularly implicated in JSRV Env-mediated autophagy inhibition.
  • Understanding this mechanism provides insights into JSRV-induced OPA pathogenesis.
Keywords:
AutophagyEnvelopeJaagsiekte sheep retrovirusOvine pulmonary adenocarcinomaSignaling pathway

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