Related Experiment Video
Updated: Mar 7, 2026
A Protocol for Explant Cultures of IDH1-mutant Diffuse Low-grade Gliomas
Published on: May 9, 2025
Finding of IDH1 R132H mutation in histologically non-neoplastic glial tissue changes surgical strategies, a case
Christian Baastrup Søndergaard1, David Scheie2, Astrid Marie Sehested3
1Department of Neurosurgery, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, 2100, København Ø, Denmark. csoend@gmail.com.
Introduction:
In 2016, the WHO classification of diffuse astrocytoma began to include isocitrate dehydrogenase (IDH) mutation in addition to histology.
Results:
We here demonstrate a case where a 14-year-old boy presented with a parietal tumor with no histological evidence of neoplasia but with an IDH1 mutation. Due to the IDH1 R132H mutation, the patient was diagnosed with diffuse astrocytoma WHO grade II and underwent successful gross total resection of this near-eloquently located tumor.
Conclusion:
This case exemplifies how inclusion of immunohistochemistry in tumor classification alters surgical strategy and might improve accuracy and time to diagnosis.
